The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 15, 1992

Filed:

Aug. 23, 1989
Applicant:
Inventors:

Gary E Hewett, Los Altos Hills, CA (US);

Steven T Mielke, Fremont, CA (US);

Judith A Blunt, Fremont, CA (US);

Assignee:

Cholestech Corporation, Hayward, CA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12M / ; C12Q / ; G01N / ;
U.S. Cl.
CPC ...
435289 ; 435 11 ; 435 28 ; 422 55 ; 422 56 ; 436164 ; 436 13 ;
Abstract

An assay device and method for determining the concentration of an analyte in a fluid sample. The device includes a porous-matrix reservoir which receives the sample to be assayed, and at least two reaction zones into which sample fluid is drawn from the reservoir. In an embodiment containing two reaction zones, a single-point standard curve which corrects for reduced-signal effects related to loss of reagent activity and linear inhibition effects, is used to determine analyte concentration. In an embodiment containing three reaction zones, a two-point standard curve which corrects for reduced-signal effects related loss of reagent activity and non-linear inhibition effects, is used to determine analyte concentration. Also disclosed is an apparatus which uses the device for automated or semi-automated analyte assay.


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