The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 03, 1992
Filed:
May. 14, 1991
Applicant:
Inventors:
John F Carpenter, Marietta, GA (US);
Kelvin G Brockbank, Marietta, GA (US);
Assignee:
Cryolife, Inc., Marietta, GA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61F / ;
U.S. Cl.
CPC ...
600 36 ; 62 62 ; 623901 ; 128D / ;
Abstract
A method is disclosed for preparing a transplantable tissue which has been cryopreserved with an intracellular cryoprotectant and then thawed. After thawing, the tissue is treated with a diluent (or eluent) solution to reduce the level of cryoprotectant in the cells to a substantially non-toxic level. This is conducted using a single dilution step. Cryoprotectants which are removable in this manner include dimethylsulfoxide, glycerol, propanediol and other compounds which penetrate the cells. The dilution step does not require end point titration and can be conducted over a time period greater than about 5 minutes.