The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 20, 1992
Filed:
Oct. 24, 1990
Applicant:
Inventors:
Lars Abrahmsen, Stockholm, SE;
Tomas Moks, Taby, SE;
Bjorn Nilsson, Sollentuna, SE;
Mathias Uhlen, Uppsala, SE;
Assignee:
KabiGen AB, Stockholm, SE;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P / ; C12P / ; C12N / ; C12N / ;
U.S. Cl.
CPC ...
435 691 ; 435 91 ; 4351723 ; 4352351 ; 4353201 ; 4352523 ; 43525233 ; 536 27 ; 530350 ; 935-9 ; 935 27 ; 935 31 ; 935 48 ; 935 56 ; 935 58 ; 935 61 ; 935 72 ; 935 73 ;
Abstract
A process for expressing proteins in Gram(-) bacteria and providing for extracellular secretion thereof, comprising the steps: a) introducing into a Gram(-) bacterium a recombinant DNA construction comprising a promoter, a signal sequence enabling translocation and processing, and a structural gene encoding the desired protein to be expressed; b) cultivating the bacterium under conditions resulting in filamentous growth; and c) recovering the extracellularly secreted protein; a recombinant DNA construction comprising: