The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 29, 1992
Filed:
Apr. 05, 1990
Hideo Kawaguchi, Hachioji, JP;
Kouji Takeuchi, Nagareyama, JP;
Tadashi Ishibashi, Kumagaya, JP;
Norio Shimizu, Sayama, JP;
Yoji Odawara, Kodaira, JP;
Hitachi, Ltd., Tokyo, JP;
Abstract
By culturing cells in an apparatus containing a first zone in which a cell suspended medium is present and a second zone in which liquid containing a physiological saline solution and/or nutrient sources is present, the first zone being separated from the second zone by a septum with characteristics, such as pores size or electrostatic charges, which impedes the cells from freely permeating through, while allowing at least one of nutrient sources, cell wastes or the product to move between the first zone and the second zone via the septum to proliferate cells in the first zone, wherein a flow rate via the septum is controlled in such a manner that the cell count moved from the first zone into the second zone via the septum does not exceed the cell count increased by cell growth in the first zone. Thus, cell culture at a high cell density can be achieved in a large scale.