The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 29, 1992
Filed:
May. 05, 1989
Applicant:
Inventors:
Shyoichiro Miyahara, Yokohama, JP;
Maki Suzuki, Yokohama, JP;
Atsunori Shindo, Kamakura, JP;
Nobumi Kusuhara, Yokohama, JP;
Nobuyoshi Makiguchi, Fujisawa, JP;
Assignee:
Mitsui Toatsu Chemicals Incorporated, Tokyo, JP;
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12N / ; C12N / ;
U.S. Cl.
CPC ...
435226 ; 435217 ; 435212 ; 435215 ;
Abstract
A method for the production of human tissue type plasminogen activator (tPa) using cells is disclosed. The method includes a supplementation of a p-aminomethyl benzoic acid derivative to a cell culture medium or a tPA producing medium and further an increase of osmotic pressure in the medium to 350 milliosmoles or more/liter. The invention provides a method for producing single-chain tPA in a high concentration and with a relatively small amount of double-chain tPA in the medium.