The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 22, 1992
Filed:
May. 16, 1990
Stephen D Gillies, Scituate, MA (US);
Abbott Biotech, Inc., Needham Heights, MA (US);
Abstract
Disclosed is a method of increasing production of proteins, e.g., human tPA, in mammalian cells which normally secrete immunoglobulins. Degradation of mRNA transcribed from recombinant DNA is decreased by decreasing the length of the untranslated region of the mRNA. The untranslated region of DNA encoding a protein of interest is altered to produce a shorter recombinant DNA having an untranslated region comprising a poly A addition signal (AATAAA) and less than about 300 nucleotide bases interposed between said poly A signal and the stop codon 3' of the coding region of the gene of interest. The mammalian cell line is transfected and cultured to produce greater amounts of the protein of interest than the same cell line transfected with the unaltered DNA.