The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 04, 1992
Filed:
May. 02, 1990
Stellan Hjerten, Uppsala, SE;
Jia-Li Liao, Uppsala, SE;
Bio-Rad Laboratories, Inc., Hercules, CA (US);
Abstract
Highly compressible chromatographic stationary phase particles such as agarose beads are made rigid to a degree suitable for use in HPLC, and nonporous to proteins by one of two procedures. The first involves shrinking the beads with the use of an organic solvent in which the agarose bead will neither dissolve nor swell to collapse the porosity, followed by crosslinking the bead surfaces inside the collapsed pores to fix the pores in their collapsed state. The second involves filling the pores (without shrinkage of the beads) with a polymerizable substance which grafts to the pore surface, and performing the graft polymerization. The invention also extends to rigid beads, which are rendered deformable to a limited degree by coating the surface with a polymer. Finally, porous rigid beads are rendered nonporous by polymerizing a polymerizable material inside the pores in the same manner as the porous compressible beads.