The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 16, 1992

Filed:

May. 03, 1988
Applicant:
Inventors:

Grace E Martin, Tarrytown, NY (US);

Robert J Kanter, Old Bethpage, NY (US);

Young R Kim, Hartsdale, NY (US);

Leonard Ornstein, White Plains, NY (US);

Hudson R Ansley, Warwick, NY (US);

Assignee:

Technicon Instruments Corporation, Tarrytown, NY (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N / ;
U.S. Cl.
CPC ...
435-724 ; 435 21 ; 435 28 ; 435 29 ; 435-72 ; 435-721 ; 435-75 ; 435-79 ; 436 63 ; 436166 ; 436172 ; 436519 ; 436521 ; 436532 ; 436548 ; 436805 ; 436825 ;
Abstract

A flow cytometry method for reproducibly detecting and counting a lymphocyte population of interest in a leukocyte suspension or whole blood sample in which the red cells are subsequently lysed. The suspension (or sample) is combined with a reagent comprising a primary antibody, either native, carrying an attached enzyme or biotin or other label, and a fixative reagent, in either order. Where the enzyme is not attached, an enzyme is coupled specifically to the primary antibody. The fixed suspension is reacted with a color-producing enzyme-cytochemical reagent. The suspension, now including stained and unstained fixed cells, is passed through a flow cytometer and the cells are characterized and counted on the basis of their light-scattering and light-absorbing properties.


Find Patent Forward Citations

Martin, Grace. (1992). Method for discriminating surface stained lymphocytes (U.S. Patent No. 5122453). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/5122453

Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile

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