The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 16, 1992
Filed:
Feb. 08, 1991
Hiroshi Yamazaki, Nepean, CA;
Burton W Blais, Ottawa, CA;
Carleton University, Ottawa, CA;
Abstract
An immunoassay device containing an immobilized antibody or antigen is provided by directly absorbing and absorbing an unmodified antibody or antigen on and within a woven or non-woven macroporous hydrophobic synthetic polymer cloth formed of a synthetic polymer selected from the group consisting of plypropylene, polyester, nylon and polyethylene. The cloth has a thickness of more than about 200 .mu.m and contains pores in the form of spaces between fibers exceeding about 20 .mu.m in diameter, and has a Frazier Air Permeability in CFM/ft.sup.2 at 0.5' H.sub.2 O of about 215 for a cloth of thickness of about 40 mils. The cloth has a large surface area for binding to an antibody or antigen and can accommodate a large volume of liquid per surface area and has minimum flow resistance. The cloth containing an immobilized antibody or antigen may be used to carry out an enzyme immunoassay by contacting the cloth with a sample containing an antigen or antibody, incubating the cloth with an enzyme-antibody conjugate and then reacting enzyme bound to the cloth with a chromogenic substrate-indicator to produce a visible color. Other immunoassay embodiments may also be carried out and a control cloth can be used such that a difference in color from that obtained with the control cloth determines the amount of antigen or antibody present in a sample.