The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 26, 1992

Filed:

Aug. 31, 1989
Applicant:
Inventor:

David J Brigati, Edmond, OK (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ;
U.S. Cl.
CPC ...
435-6 ; 4351723 ; 436501 ; 536 187 ; 536 21 ; 935 78 ;
Abstract

Probe cocktails contain a nucleic acid probe and a sulfated polysaccharide as a volume excluding polymer to speed up hybridization rates. Exemplary sulfated polysaccharides are chondroitin sulfate A and C, which can be extracted from cartilage and similar tissues. Compared to synthetic anionic polymers such as dextran sulfate, the probe cocktails can have reduced viscosities and surface tensions, as well as reduced water loss when the cocktail is heated to denature DNA before rehybridization. The reduced viscosity and surface tension are of particular value when entering and exiting narrow spaces, as in some automated analyzers and in capillary gap methodologies.


Find Patent Forward Citations

Loading…