The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 12, 1992
Filed:
Feb. 23, 1990
Snow Brand Milk Products Co., Ltd., Sapporo, JP;
Abstract
A novel tissue plasminogen activator having the following characteristics: molecualr weight of 65,000-72,000 Daltons as measured by SDS-PAGE electrophoresis using at 7.5% agarose gel; plasminogen activator specific activity of about 10.4.times.10.sup.4 IU/mg, wherein specific activity is defined as the ratio of fibrinolytic activity of purified t-PA measured on fibrin-agarose plates to milligrams of protein; about 83.1% absorption of t-PA by a fibrin-Sepharose column when applied; binds to a Concanavalin A column when applied; the fibrinolytic activity is substantially undiminished by heating at 60.degree. C. for 60 minutes or 95.degree. C. for 5 minutes relative to unheated t-PA; unreactive with polyclonal antisera raised against urokinase; the fibrinolytic activity is substantially stable at pH 5-10; exhibits fibrinolytic activity at pH 7.5-9.0 and temperature 39.degree.-41.degree. C.; a Km value of about 1.16.times.10.sup.-3 mol/liter and a V.sub.max of about 11.7.times.10.sup.-8 mol/liter for substrate S-2288; and fibrinolytic activity is inhibited by Co.sup.2+ Zn.sup.2+, Cd.sup.2+, Hg.sup.2+, Ni.sup.2+ and Cu.sup.2+.