The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 14, 1992

Filed:

Mar. 13, 1990
Applicant:
Inventors:

Hitoshi Kondo, Uji, JP;

Kazuhiro Matsui, Tsuruga, JP;

Hiroshi Suzuki, Yachiyo, JP;

Assignees:

Unitika Ltd., Hyogo, JP;

Iatron Laboratories, Inc., Tokyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ; G01N / ;
U.S. Cl.
CPC ...
435 25 ; 435-4 ; 435 18 ; 435 28 ; 436 97 ; 436903 ;
Abstract

Disclosed is a process for measuring an amount of all bilirubins in a specimen, comprising deconjugating conjugated bilirubins with a reagent comprising an enzyme capable of deconjugation to form the unconjugated bilirubin, and determining an amount of the unconjugated bilirubin in the specimen, and a reagent therefor. The present invention also provides a process for measuring an amount of the unconjugated bilirubin in a specimen, comprising oxidizing and eliminating conjugated bilirubins in the specimen with an oxidizing agent capable of oxidizing bilirubins, in the presence of a metal ion forming a complex with the bilirubins and a surfactant at pH 6.5 or less, and determining an amount of the unconjugated bilirubin in the specimen, and a reagent therefor.


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