The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 31, 1992

Filed:

Oct. 03, 1989
Applicant:
Inventors:

Thomas W Rademacher, Oxford, GB;

Mark R Wormald, Oxford, GB;

Raj B Parekh, Oxford, GB;

Christopher J Edge, Oxford, GB;

Raymond A Dwek, Oxford, GB;

Assignee:

Monsanto Company, St. Louis, MO (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ;
U.S. Cl.
CPC ...
435 18 ; 435 22 ; 435101 ;
Abstract

A method of oligosaccharide sequencing in which the components are determined essentially simultaneously is disclosed which comprises a series of steps as follows: A. Placing an identifying label on the reducing terminal residue of the oligosaccharide to be sequenced, B. Dividing said oligosaccharide into a plurality of separate portions of known integer amounts, C. Treating each said portion with a different reagent mix to thereby provide a series of reaction mixtures, D. Pooling known integer amounts of the products from each separate reaction mixture to give a product pool, E. Performing an analysis on said product pool which measures the molar proportions of the reaction products, and F. Reconstructing or identifying the starting oligosaccharide from the molar prevalence of said reaction products.


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