The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 24, 1992

Filed:

Jul. 16, 1990
Applicant:
Inventors:

David Bar-or, Denver, CO (US);

Clive Solomons, Denver, CO (US);

Assignee:

Diagnostic Markers, Inc., Englewood, CO (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C12Q / ; C12Q / ; C12Q / ;
U.S. Cl.
CPC ...
435 28 ; 435 34 ; 435 19 ; 435 30 ; 435 29 ;
Abstract

A method for the detection of a fungal infection within 1-2 hours comprises the steps of: (a) contacting a sample suspected of containing a peroxidase-containing fungus with a mixture which comprises (i) a peroxide from hydrogen peroxide and addition compounds thereof containing latent hydrogen peroxide; (ii) a scavenger to remove heavy metals which may potentially decompose said peroxide catalytically; (iii) an alkaline buffer solution having the capacity to maintain the pH at a value within the range of 9.5 to 14; and (iv) a colorless oxidizable substrate (such as tyrosine, .beta.-(3,4-dihydroxyphenyl)-.alpha.-alamine (DOPA) or caffeic acid), which on reaction with oxygen at said pH value gives a visibly colored oxidation product; and (b) observing whether a color develops indicating the presence of peroxidase-containing fungus in the sample. Also included is a test kit for carrying out the method of the invention.


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