The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 17, 1992
Filed:
Jun. 04, 1990
Applicant:
Inventor:
David A DeBonville, Beverly, MA (US);
Assignee:
AutoGen Instruments, Inc., Beverly, MA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P / ; C12N / ;
U.S. Cl.
CPC ...
435 91 ; 4351721 ; 4351723 ; 435259 ; 536 27 ; 935 19 ; 935 20 ; 935 21 ;
Abstract
An improved method for isolating and purifying nucleic acid from cell culture media contemplating adding to resuspended cell solutions a lysing solution and neutralizing/deproteinating agent without mechanical mixing, followed by centrifugation to partially pellet cellular debris, mixing the solution to complete the reaction, and additional centrifugation to fully pellet the debris.