The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 04, 1992

Filed:

Aug. 28, 1990
Applicant:
Inventors:

Koji Ushizawa, Tokyo, JP;

Takae Shigihara, Tokyo, JP;

Maki Akemichi, Tokyo, JP;

Toshiyuki Akimoto, Tokyo, JP;

Senkichi Nagasaki, Tokyo, JP;

Miyoshi Hirata, Tokyo, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ; C12Q / ;
U.S. Cl.
CPC ...
435 26 ; 435 25 ;
Abstract

A quantitative analysis of 3-oxo-5.beta.-steroid is carried out by acting 3-oxo-5.beta.-steroid.DELTA..sup.4 -dehydrogenase on a sample in the presence of a reducing chromophoric agent, and measuring the optical density of the chromophoric substance thereby produced. A typical reducing chromophoric agent is a tetrazolium compound. The invention also provides a reagent for the quantitative analysis of 3-oxo-5.beta.-steroid comprising 3-oxo-5.beta.-steroid.DELTA..sup.4 -dehydrogenase and a tetrazolium compound. The analysis and reagent provide a simple and reliable method for a liver function test.


Find Patent Forward Citations

Loading…