The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 21, 1992
Filed:
Dec. 15, 1988
Kenneth A Cruickshank, Naperville, IL (US);
Amoco Corporation, Chicago, IL (US);
Abstract
This invention encompasses diagnostic reagents comprising a polynucleic acid probe having a specific binding sequence and having one or more cytidines outside of the specific binding sequence. The polynucleic acid probe is bound to an amino functionalized solid support by a bisulfite mediated transamination between a cytidine and an amino group on the solid support. When the specific binding sequence contains cytidine, the cytidine is replaced with 5-methylcytidine which has essentially the same binding capacity and yet does not participate in transamination reactions. The invention encompasses compositions of the polynucleic acid probe as well as test kits including the above identified reagent and assays utilizing the above reagent. This invention is useful in detecting viruses, fungi and bacteria in test samples such as body fluids and food samples as well as detecting DNA or RNA sequences in mammalian cells.