The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 07, 1992

Filed:

Mar. 21, 1990
Applicant:
Inventors:

Anthony H Carr, Bedford, GB;

Robert A Badley, Bedford, GB;

Ian Jobling, Rushden, GB;

Thomas J Sands, Wellingborough, GB;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ; C12Q / ; C12Q / ; C12M / ;
U.S. Cl.
CPC ...
435 32 ; 435 30 ; 435 34 ; 435296 ; 435805 ; 435810 ;
Abstract

A test for microbial growth is carried out by culturing a microorgnism to be tested in a lliquid culture medium containing an enzymatically hydrolyzable fluorogenic substrate for a period of less than about 7 hours, measuring fluorescence of the culture medium resulting from hydrolysis of the fluorogenic substrate by growth of the microorganism, continuing culturing of the microorganism in the culture medium for an additional period, and measuring microorganism growth by a non-fluorescent method as a confirmatory test of growth determined from fluorescence. Preferably, the additional culture period is overnight and the non-fluorescent method is by visual inspection. The culture medium may be in the wall of a microtitre plate, and the culture medium can contain an antibiotic to test for sensitivity of a microorganism to the antibiotic or to test for minimum inhibitory concentration of the antibiotic.


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