The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 31, 1991

Filed:

Jan. 13, 1989
Applicant:
Inventors:

Frances S Eigler, Potomac, MD (US);

Jacque Georger, Springfield, VA (US);

Suresh K Bhatia, Alexandria, VA (US);

Jeff Calvert, Burke, VA (US);

Lisa C Shriver-Lake, Springfield, VA (US);

Reinhard Bredehorst, Washington, DC (US);

Assignee:

Other;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; G01N / ; G01N / ;
U.S. Cl.
CPC ...
435176 ; 435181 ; 436524 ; 436527 ; 436532 ; 530811 ; 530816 ;
Abstract

Active agents such as proteins are covalently immobilized on substrates carrying hydroxyl groups. A silane is bound to the substrate and coupled to a heterobifunctional crosslinker at one functional group leaving a free functional group, different than the first group, to which a protein is bound while retaining high protein functionality. Preferably, the silane has a functional group which reacts with the hydroxyl group of the substrate and a thiol terminal group which reacts with a functional group of a heterobifunctional crosslinking agent which contains a succinimide group that reacts with an amino group of the active agent. Bound active agents such as proteins are useful as biosensors or reactants in a variety of applications including bioassays.


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