The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 12, 1991

Filed:

Sep. 12, 1989
Applicant:
Inventors:

Nobuyoshi Ebata, Tokyo, JP;

Yuko Tanaka, Tokyo, JP;

Akiko Negishi, Tokyo, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N / ; G01N / ;
U.S. Cl.
CPC ...
436164 ; 422 61 ; 436 86 ; 436 94 ; 436169 ; 436905 ;
Abstract

An improved silver staining technique is disclosd for the detection of a biocomponent contained in a support medium. The technique includes fixing the support medium with a fixing agent, pretreating the thus-fixed support medium with a sensitizer, treating the resultant support medium with a silver staining solution and then developing the thus-treated support medium with a reducing agent. In one aspect of the invention, thiourea is incorporated in either one or both of the fixing agent and sensitizer. In another aspect of the invention, thiosulfate is contained in the reducing agent. According to the invention, improved stainability and contrast are obtained. Also disclosed is a silver staining kit.


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