The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 05, 1991

Filed:

Aug. 04, 1989
Applicant:
Inventors:

Colin J Suckling, Glasgow, GB;

Peter J Halling, Glasgow, GB;

Grant A Johnston, West Kilbride, GB;

Linda Brown, Glasgow, GB;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ; C12Q / ; C12Q / ; C07D / ;
U.S. Cl.
CPC ...
435-4 ; 435 18 ; 435 19 ; 435 23 ; 435 39 ; 435135 ;
Abstract

A process for performing an enzyme-catalyzed reaction in a reaction mixture comprising two phases, a first aqueous phase containing an enzyme which is not accessible and a second substantially non-aqueous phase containing a non-water miscible solvent wherein a compound derived from fluorescein or a substituted fluorescein is present for the purpose of measuring aqueous pH by monitoring spectroscopic properties. Novel compounds derived from fluorescein or a substituted fluorescein which may be used in the process and a method for producing some of the novel compounds are also claimed.


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