The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 15, 1991
Filed:
Apr. 07, 1989
Frieder K Hofmann, Oceanside, CA (US);
Wolfgang J Wrasidlo, LaJolla, CA (US);
Brunswick Corporation, Skokie, IL (US);
Abstract
An enzyme reactor system is provided based on the entrapment of a coenzyme-requiring enzyme, a coenzyme, and a regeneration enzyme in a hydrogel layer coated on a support, and confined by an ultraporous thin film semipermeable membrane. The diffusion barrier confines the coenzyme-requiring enzyme, coenzyme, and regeneration enzyme but lets substrate and reaction products, exclusive of coenzyme, diffuse freely into and out of the hydrogel layer. In an alternate embodiment, the support is formed of an ultraporous thin film semipermeable membrane on a microporous or macroporous support, through which the reaction products, exclusive of coenzyme, can diffuse freely, but through which neither coenzyme-requiring enzyme, coenzyme, regeneration enzyme, nor substrate can pass. In this embodiment, the product is recovered in high purity, free of substrate, coenzyme-requiring enzyme, coenzyme, and regeneration enzyme. A constant supply of coenzyme for the enzyme reaction is assured by initially charging the hydrogel layer with an adequate supply of coenzyme. The problem of coenzyme depletion is solved by including a second enzyme in the hydrogel layer which regenerates the used coenzyme, thus recycling it. The substrates for both the primary and regeneration reactions are supplied in the circulating solvent. Reaction products, exclusive of coenzyme, are carried away by the solvent.