The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 15, 1991
Filed:
Jun. 13, 1988
Applicant:
Inventors:
Leon W Terstappen, Los Altos, CA (US);
Michael R Loken, Palo Alto, CA (US);
Virendra O Shah, Santa Clara, CA (US);
Assignee:
Becton, Dickinson and Company, Franklin Lakes, NJ (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C12Q / ; C12Q / ; G01N / ;
U.S. Cl.
CPC ...
435-6 ; 435-71 ; 435-72 ; 435 29 ; 435 34 ; 436 63 ; 436 172 ; 436800 ;
Abstract
A method is disclosed for the discrimination between intact and damaged cells in a sample, and more particularly, the method uses a vital nucleic acid dye to selectively stain intact versus damaged cells which then may be counted and sorted by flow cytometry means. The method also may be used in conjunction with fluorescently labelled monoclonal antibodies to simultaneously identify cellular antigens.