The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 03, 1991
Filed:
Feb. 23, 1990
Kiell Bertheussen, Eidkjosen, NO;
Medi-Cult A/S, , DK;
Abstract
The present invention discloses a serum-free growth medium with iron-enhancing cell culture characteristics provided by a transferrin-replacing substance. The transferrin replacement preferably comprises an iron-chelate, aurintricarboxylic acid and alkali-metal-EDTA. More particularly, the iron-chelate comprises a mixture of citrate/citric acid and Fe/EDTA. Trace elements and growth factors are also included in the disclosed medium. The growth medium further comprises base culture media. Most preferably, the base media comprise a mixture of RPMI 1640, HEPES and NaHCO.sub.3. The growth medium together with a culture of hybridoma eukaryotic cells may be used for quality testing of other growth media, optionally together with a preferred hybridoma cell line from P.sub.3 U.sub.1 tumor cells and B-lymphocytes. This test procedure is provided by way of a containerized kit which includes the serum-free medium and the most preferred fast-growing eukaryotic cell line, hybridoma ECACC 86 112001.