The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 06, 1991
Filed:
Dec. 02, 1987
Thomas J Burr, Geneva, NY (US);
Andrew L Bishop, Geneva, NY (US);
Veronica L Mittak, Geneva, NY (US);
Cornell Research Foundation, Inc., Ithaca, NY (US);
Abstract
A monoclonal antibody specific to Agrobacterium tumefaciens biovar 3. A method of diagnosing Agrobacterium tumefaciens biovar 3 associated grapevine disease comprising: (1) culturing bacteria from grapevine tissue suspected of being infected with Agrobacterium tumefaciens biovar 3; (2) reacting the bacteria with a monoclonal antibody specific to Agrobacterium tumefaciens biovar 3 under conditions sufficient to form an antigen-antibody complex between antigens specific to Agrobacterium tumefaciens biovar 3 and the monoclonal antibody; and (3) detecting the presence of the antigen-antibody complex. A method for diagnosing Agrobacterium tumefaciens biovar 3 associated grapevine disease from crown gall tissue comprising: (1) preparing separate suspensions of ground gall tissue to be diagnosed and of ground wood of the same cultivar as a control; (2) separately reacting specific to Agrobacterium tumefaciens biovar 3 under conditions sufficient to form an antigen-antibody complex between antigens specific to Agrobacterium tumefaciens biovar 3 and the monoclonal antibody; (3) assaying for the presence of the antigen-antibody complex ; and (4) comparing the assay results for the gall tissue to be diagnosed to the assays results for the wood control. A method for diagnosing agrobacterium tumefaciens biovar 3 associated grapevine disease from nonsymptomatic grapevine cuttings comprising: (1) separately flushing fluid through cuttings to be diagnosed and through uninfected control cuttings; (2) separately reactign the fluid flushed through the cuttings with monoclonal antiody specific to agrobacterium tumefaciens biovar 3 under conditions sufficient to form an antigen-antibody complex between antigens specific to Agrobacterium tumefaiens biovar 3 and the monoclonal antibody; (3) assaying for the presence of the antigen-antibody complex; and (4) comparing the assay results for said cuttings to be diagnosed to the assay results for the control cuttings. A hybridoma that secretes the above-described monoclonal antibody and a method of producing the hybridoma.
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