The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 09, 1991

Filed:

Dec. 14, 1989
Applicant:
Inventors:

Christopher J Power, Boulder, CO (US);

Ebrahim Firoozabady, Madison, WI (US);

Assignee:

Lubrizol Genetics, Inc., Wickliffe, OH (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ;
U.S. Cl.
CPC ...
4352405 ; 43524049 ; 43524054 ;
Abstract

A method for regeneration of sunflower plants via shoot induction from cotyledon explants prepared from non-germinated sunflower embryos is described. Several methods for preparing explants by dissection of mature and immature fully-formed embryos are provided. Explants are prepared to insure that substantially all of the induced shoots derive from single cotyledon cells. Appropriately prepared explants are cultured on a shoot induction medium containing a combination of BA and NAA. Induced shoots are rooted and the resulting plantlets are grown into mature sunflower plants. This regeneration method is efficient, rapid and employs a convenient explant source, sunflower seed. This regeneration method is particularly useful in combination with methods for introduction of foreign DNA into sunflower cells and tissue.


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