The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 18, 1991

Filed:

Jun. 15, 1987
Applicant:
Inventors:

Glenn B Collins, Lexington, KY (US);

David F Hildebrand, Lexington, KY (US);

Paul A Lazzeri, Lexington, KY (US);

Thomas R Adams, North Stonington, CT (US);

Wayne A Parrott, Versailles, KY (US);

Lynn M Hartweck, Lexington, KY (US);

Assignees:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ;
U.S. Cl.
CPC ...
4351723 ; 43524049 ; 4352405 ; 43524054 ; 935 67 ;
Abstract

A method for somatic embryogenesis of soybean, (Glycine max), Glycine soja and other Glycine species is provided using immature cotyledon tissue, preferably with the embryonic axis removed, comprising culturing said tissue on a medium containing auxin, preferably NAA at a concentration of at least about 15 mg/l. A further method for such somatic embryogenesis is provided wherein the culture medium contains a synergistically acting lowered carbohydrate and auxin concentration. Particularly embryogenic cells of such tissue are identified and improved maceration methods for contacting such cells with regeneration and transformation media are disclosed. Methods for transforming somatic tissue from soybean and other Glycine species are also provided. Whole, fertile, transformed plants are obtained.


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