The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 26, 1991
Filed:
May. 19, 1988
Applicant:
Inventors:
Joan E Brooks, Beverly, MA (US);
Laura A Sznyter, Beverly, MA (US);
Assignee:
New England Biolabs, Inc., Beverly, MA (US);
Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12N / ;
U.S. Cl.
CPC ...
4351723 ; 435199 ; 4352523 ; 4353201 ; 435193 ; 43525233 ; 536 27 ; 935 29 ; 935 73 ; 935 80 ; 935 82 ;
Abstract
The present invention is directed to a method for cloning and producing the Eag I restriction endonuclease by (1) introducing the restriction endonuclease gene from E. agglomerans ATCC into a host whereby the restriction gene is expressed; (2) fermenting the host which contains the vector encoding and expressing the Eag I restriction endonuclease, and (3) purifying the Eag I restriction endonuclease from the fermented host which contains the vector encoding and expressing the Eag I restriction endonuclease activity.