The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 22, 1991

Filed:

Mar. 14, 1990
Applicant:
Inventors:

Michael L Berman, Potomac, MD (US);

P Scott Meissner, Barnesville, MD (US);

William P Sisk, Kensington, MD (US);

Assignee:

Akzo N.V., Arnhem, NL;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12P / ; C12N / ; C07H / ;
U.S. Cl.
CPC ...
4351723 ; 435 91 ; 4353201 ; 536 27 ; 935-8 ; 935 18 ; 935 77 ; 935 80 ;
Abstract

A bacteriophage directional cloning vector containing a multiple cloning site, a method for preparing cDNAs that can be cloned in a unique orientation and a bifunctional oligodeoxynucleotide linker, which creates a unique restriction site when ligated to the 3' end of cDNA fragments. The simple and efficient cDNA cloning system reduces the amount of RNA and effort required for the preparation of large directionally cloned libraries by providing a unique vector that accepts cDNA fragments in a unique 5'-3' orientation and by providing a unique bifunctional linker that facilitates the preparation of cDNA fragments that can be ligated directionally into the vector.


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