The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 09, 1990

Filed:

Jun. 03, 1987
Applicant:
Inventors:

Kirk J Hayenga, San Bruno, CA (US);

Virgil B Lawlis, Pacifica, CA (US);

Bradley R Snedecor, Brisbane, CA (US);

Assignee:

Genentech, Inc., San Francisco, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A23C / ; A23C / ; C12N / ; C12N / ;
U.S. Cl.
CPC ...
426 36 ; 435 691 ; 435 712 ; 4351723 ; 435220 ; 435222 ; 435224 ; 4352523 ; 43525231 ; 43525233 ; 935 14 ; 935 38 ; 935 60 ; 935 61 ; 935 73 ; 935 74 ;
Abstract

Cheese is prepared using rennin obtained from insoluble refractile bodies of a recombinant microbial host cell. The rennin is obtained by rupturing the recombinant hose cell, isolating and solubilizing the insoluble refractile bodies, and recovering active rennin. Recombinant techniques involve preparing cDNA corresponding to the coding sequence for calf rennin, introducing into an expression vector and expressing in a host cell. As much as 200 mg rennin per liter of culture may be recovered. Prorennin or preprorennin may be produced and rennin derived therefrom.


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