The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 02, 1990
Filed:
Dec. 12, 1988
Applicant:
Inventors:
Assignee:
Toyo Jozo Co., Ltd., Shizuoka, JP;
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ;
U.S. Cl.
CPC ...
536 27 ; 4352523 ; 43525231 ; 43525233 ; 435 691 ; 935 69 ; 935 73 ; 935 74 ; 935 60 ; 935 14 ;
Abstract
DNA having a base sequence encoding a specified amino acid sequence of a polypeptide constituting an L-.alpha.-glycerophosphate oxidase (GPO) is disclosed. A polypeptide having the specified amino acid sequence can be produced by inserting the DNA into a plasmid, culturing a transformant produced by introducing this plasmid into a host microorganism which is selected from microorganisms belonging to Escherichia coli, Bacillus subtilis, or Saccharomyces cerevisiae. The GPO is useful for the quantitative analysis of triglyceride, glycerine, etc., and the determination of lipase activity, when used in combination with other enzymes such as lipase, glycerol kinase, or the like, or with an ATP reagent.