The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 28, 1990

Filed:

Jun. 08, 1987
Applicant:
Inventors:

Robert T Belly, Ithaca, NY (US);

Patricia M Scensny, Rochester, NY (US);

Annie L Wu, Penfield, NY (US);

Chung-yuan Chen, Rochester, NY (US);

Assignee:

Eastman Kodak Company, Rochester, NY (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ; C07D / ;
U.S. Cl.
CPC ...
435 18 ; 435-4 ; 435-7 ; 435 14 ; 435 19 ; 435 21 ; 435 22 ; 435 23 ; 435 24 ; 558 37 ; 558194 ; 530408 ; 530409 ; 530410 ; 536-41 ; 536 174 ; 544 31 ; 544337 ; 544347 ; 552296 ; 552298 ; 548368 ;
Abstract

Hydrolyzable substrates comprise blocked moieties which, when cleaved from the substrate during hydrolysis, provide electron transfer agents. The released electron transfer agents can be recycled between a reductant and a reducible compound that upon reduction provides a detectable species. Alternatively, they can be recycled between an oxidant and an oxidizable compound that upon oxidation provides a detectable species. These substrates are useful in analytical compositions, elements and methods for the determination of hydrolytic analytes, such as hydrolytic enzymes or biological cells containing such enzymes.


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