The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 14, 1990
Filed:
Feb. 06, 1987
Applicant:
Inventors:
Salvador E Luria, Lexington, MA (US);
Joan L Suit, Cambridge, MA (US);
Jennifer A Jackson, Reading, MA (US);
Assignee:
Repligen Corporation, Cambridge, MA (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12N / ; C12P / ;
U.S. Cl.
CPC ...
4352528 ; 435 691 ; 435 697 ; 435 698 ; 435 712 ; 43525233 ; 435259 ; 435320 ; 435849 ; 935 47 ; 935 48 ;
Abstract
The invention concerns novel and useful mutant microbes which are capable of releasing substantial amounts of any of several periplasmic or recombinant proteins into the culture medium when carrying an expressed Kil gene. Though E. coli are exemplified, the invention is broadly applicable to the making of mutants of other microbes, for example, Salmonella, Klebsiella, and Rhizobium. A key feature of the invention is the use of a novel selection procedure employing a plasmid comprising the kil gene.