The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 14, 1990
Filed:
Mar. 17, 1988
Applicant:
Inventors:
Michael Piatak, Jr, Walnut Creek, CA (US);
Walter J Laird, Pinole, CA (US);
Julie A Lane, Oakland, CA (US);
Assignee:
Cetus Corporation, Emeryville, CA (US);
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12P / ; C12N / ; C12N / ; C07H / ;
U.S. Cl.
CPC ...
436 68 ; 4351721 ; 4351723 ; 4352523 ; 435320 ; 536 27 ; 935 11 ; 935 46 ; 935 72 ;
Abstract
A system for expression of coding sequences for desired heterologous proteins in procaryotic hosts whereby the protein is produced intracellularly in soluble, biologically active form, is disclosed. The expression is obtained by ligation of the coding sequence downstream of, and proximally to, but out of reading frame with, the terminated leader encoding sequence for a secreted bacterial protein, such as alkaline phosphatase. The resultant proteins are influenced by the leader sequence codons to effect the desired three-dimensional conformation, but not to effect secretion.