The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 12, 1990
Filed:
Jan. 28, 1987
Applicant:
Inventor:
Daniel T Connolly, Manchester, MO (US);
Assignee:
Monsanto Company, St. Louis, MO (US);
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ; C12Q / ;
U.S. Cl.
CPC ...
435 29 ; 435 21 ;
Abstract
A method is disclosed for determining the number of cells in cell culture which comprises lysing the cells and incubating the cell lysate with p-nitrophenyl phosphate for a predetermined period of time at acid pH and at 35.degree.-38.degree. C., and then measuring the color development at 400 to 420 nanometers and comparing with a control. The method is accurate, sensitive, and well-suited for automation, and thereby provides for the rapid sampling of large numbers of cell culture where the cells are grown under very sparse conditions.