The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 05, 1990
Filed:
Mar. 25, 1988
Applicant:
Inventors:
Robert Halenbeck, San Rafael, CA (US);
Flint Smith, Oakland, CA (US);
Michael Kunitani, Oakland, CA (US);
Assignee:
Cetus Corporation, Emeryville, CA (US);
Primary Examiner:
Int. Cl.
CPC ...
C07K / ;
U.S. Cl.
CPC ...
530351 ; 530402 ; 530408 ; 530409 ; 530410 ; 530412 ; 530416 ; 530417 ; 530422 ; 530424 ; 530825 ; 435 6952 ;
Abstract
A process for recovering substantially pure rIL-2 from transformed microorganisms in which the cells are disrupted, impure insoluble rIL-2 is separated from the bulk of the cellular components, the separated impure rIL-2 is solubilized and partially purified in a reduced form, the solubilized rIL-2 is oxidized, the oxidized rIL-2 is purified to clinically acceptable levels, and the oxidized, purified IL-2 is denatured by placing it into a solution of a chaotropic agent, solids are removed from the solution and rIL-2 is renatured from the solution.