The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 15, 1990
Filed:
Mar. 14, 1986
Applicant:
Inventor:
Jen-chang Hsia, Concord, CA;
Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01D / ;
U.S. Cl.
CPC ...
210635 ; 210656 ; 530385 ; 530413 ; 530417 ;
Abstract
The invention disclosed relates to a method for the purification of hemoglobin by the technique of affinity chromatography. Contrary to current belief, the binding of oxygen and selected polyanions to hemoglobin is not mutually exclusive. The novel method comprises immobilizing a polyanion which specicically binds hemoglobin via its polyanion binding site, on a chromatographic gel and passing the hemoglobin containing solution or mixture through the gel. The hemoglobin is thus retained in the gel, while impurities are eluted from the gel. The novel method is also applicable to the separation of unmodified hemoglobin from a liquid reaction mixture containing modified and unmodified hemoglobin.