The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 17, 1990
Filed:
Jul. 17, 1987
Asao Murai, Kawasaki, JP;
Masao Yamamoto, Tokyo, JP;
Sanraku Incorporated, Tokyo, JP;
Abstract
The present invention is directed to a method of quantitative assay for a vitamin B.sub.12 -containing substance which comprises culturing marine methanol-utilizing bacteria having B.sub.12 auxotrophy in a medium for quantitative assay of the bacteria containing pyrroloquinoline-quinone and a polyoxyethylene sorbitan fatty acid ester and, quantitatively determining vitamin B.sub.12 in the vitamin B.sub.12 -containing substance as a function of the degree of growth thereof and, to a reagent for the B.sub.12 -containing substance comprising a kit for combination of an ampule or vial having sealed therein a dry viable bacteria composition of a marine methanol-utilizing bacteria for inoculation and an ampule or vial having sealed therein a medium for the bacteria containing pyrroloquinoline-quinone and a polyoxyethylene sorbitan fatty acid ester, or in addition thereto, further an ampule or vial having sealed therein a standard dilution of vitamin B.sub.12 having a serial concentration.