The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 10, 1990

Filed:

May. 29, 1985
Applicant:
Inventors:

Jan Markussen, Herlev, DK;

Niels Fiil, Copenhagen, DK;

Mogens T Hansen, Olstykke, DK;

Kjeld Norris, Birkerod, DK;

Gustav Ammerer, East Seattle, WA (US);

Lars Thim, Gentofte, DK;

Hans O Voigt, Lyndby, DK;

Assignee:

Novo Industri A/S, Bagsvaerd, DK;

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12N / ; C12P / ;
U.S. Cl.
CPC ...
530303 ; 4351723 ; 435 694 ; 435320 ; 43525233 ; 435942 ; 435256 ; 536 27 ; 935 13 ; 935 69 ;
Abstract

Human insulin precursors containing the peptide chain B(1-29)-A(1-21) of human insulin and derivatives thereof with a bridging chain connecting the carboxyl terminus of the B(1-29)-chain with the amino terminus of the A(1-21)-chain are prepared by culturing a yeast host transformed with a replicable expression vehicle capable of expressing a DNA-sequence encoding the insulin precursor. The bridging chain is preferably relatively short and contains preferably from 2 to 8 amino acid residues. The bridging chain must not contain two adjacent basic amino acid residues (Lys or Arg) and has one Lys or Arg connected to the amino terminus of the A(1-21)-chain. Human insulin is prepared from the insulin precursors by in vitro conversion.


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