The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 13, 1990

Filed:

Jul. 31, 1985
Applicant:
Inventors:

Randolph T Hatch, Wellesley, MA (US);

Keith C Backman, Bedford, MA (US);

Assignee:

Biotechnica International, Inc., Cambridge, MA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P / ; C12P / ; C12N / ; C12N / ;
U.S. Cl.
CPC ...
435108 ; 435-3 ; 435 41 ; 435 691 ; 4351723 ; 435183 ; 4352523 ; 435320 ; 935 29 ; 935 43 ; 935 56 ; 935 73 ;
Abstract

A method of continuous product formation using at least two continuous fermentation units and a microorganism capable of being induced, in response to environmental conditions, to undergo a genetic alteration from a state favoring microorganism growth to a state favoring product production by the microorganism. The first continuous fermentation unit is maintained at environmental conditions selected to favor growth of the microorganism and to be nonpermissive for the genetic alteration. The microorganism is grown continuously in the first unit, and a portion of the growing microorganism cell mass is transferred via connecting means to the second continuous fermentation unit. Either the connecting means or the second unit is maintained at second environmental conditions selected to effect the genetic alteration. The altered microorganism is cultured in the second unit. Exudate from this second fermentor (containing microorganism mass and medium) is continually removed and the product which is present, either in the microorganisms themselves or the medium surrounding them, is extracted.


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