The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 06, 1990

Filed:

Mar. 20, 1986
Applicant:
Inventors:

Charles L Hershberger, New Palestine, IN (US);

Jeffrey L Larson, Indianapolis, IN (US);

Patricia A Reynolds, Indianapolis, IN (US);

Assignee:

Eli Lilly and Company, Indianapolis, IN (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C07H / ;
U.S. Cl.
CPC ...
4352523 ; 4351723 ; 43525233 ; 43525235 ; 435320 ; 536 27 ; 935-6 ; 935 29 ; 935 73 ; 935 75 ;
Abstract

The present invention discloses an .about.1.4 kb Bc1I-Sau3A restriction fragment containing the minimal replicon of the Streptomyces coelicolor plasmid SCP2*. This minimal replicon was deduced from plasmid derivatives constructed by in vitro deletions and has been identified as the smallest, self-replicating segment of the SCP2* plasmid. The minimal replicon specifies an ultrahigh level of plasmid DNA (more than 1000 fold increase from the basic replicon) when vectors containing this sequence are transformed into Streptomyces lividans. This replicon can be used to construct smaller, more efficient recombinant DNA shuttle vectors to clone DNA into streptomycetes.


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