The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 09, 1990
Filed:
Nov. 03, 1987
Kenichi Watanabe, Kudamatsu, JP;
Tamotu Simada, Akishima, JP;
Keiichi Nagai, Higashiyamato, JP;
Jirou Tokita, Kokubunji, JP;
Ryusei Nakano, Kokubunji, JP;
Tomoaki Sumitani, Hachioji, JP;
Hitachi, Ltd., Tokyo, JP;
Abstract
An instrument for determination of the base sequence of deoxyribonucleic acid (DNA) which is designed to dectect DNA fragments in course of gel electrophoresis in real time to determine the base sequence of DNA, and comprises a gel electrophoretic panel part wherein DNA fragments labeled with a radioisotope are supplied to four places in a gel for four kinds of complementary strand synthesis reaction systems, respectively, and subjected to electrophoresis to form an electrophoretic pattern of the DNA fragments, and a detection part for detecting the electrophoretic pattern of the DNA fragments provided at a predetermined position in said gel electrophoretic panel part so as to have a position resolving power in a direction perpendicular to the direction of electrophoretic migration of the DNA fragments, and which instrument has a means for making the temperature of the gel in the vicinity of the detection part for detecting the electrophoretic pattern of the DNA fragments higher than that on the DNA fragment supply part side. By virtue of the provision of said means, the distance between two adjacent DNA bands similar to each other in molecular weight can be elongated, the resolving power for DNA bands can be improved, and the base sequence of nucleic acid can be determined with high precision.