The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 14, 1989
Filed:
Feb. 08, 1988
Istvan Resli, Debrecen, HU;
Tibor Takacs, Debrecen, HU;
Sandor Damjanovich, Debrecen, HU;
Rezso Gaspar, Debrecen, HU;
Lajos Tron, Debrecen, HU;
Janos Szollosi, Debrecen, HU;
Janos Matko, Debrecen, HU;
Innofinance Altalanos Innovacios Penzintezet, Budapest, HU;
Abstract
The invention relates to a process and an equipment for the rapid determination of the spermium count of sperm samples and/or the ratio of the living spermia. The process comprises dissolving the fluorescent dyestuff propidium iodide in a buffer and measuring the intensity (F.sub.o), then adding the sperm sample and, after measurement of the intensity (F.sub.1) and addition of a cytoplasm membrane-permeabilizing agent, determining the intensity (F.sub.2), then adding a buffer and a permeabilizing agnet to the buffer and measuring the intensity (F.sub.3) and subsequently the intensity (F.sub.4) of the pure buffer and finally the intensity (F.sub.5) of the sperm mixture added to the buffer, and calculating the cell concentration by using the formula: ##EQU1## wherein .alpha. is the multiplication product from the slope of the calibration curve with the predilution ratio; and calculating the living cell ratio by using the formula: ##EQU2##