The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 17, 1989

Filed:

Aug. 26, 1985
Applicant:
Inventor:

S Richard Jaskunas, Indianapolis, IN (US);

Assignee:

Eli Lilly and Company, Indianapolis, IN (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C07H / ; C12P / ;
U.S. Cl.
CPC ...
43525233 ; 435320 ; 435170 ; 435849 ; 4351723 ; 536 27 ; 935-8 ; 935 29 ; 935 41 ; 935 42 ; 935 43 ; 935 45 ; 935 47 ; 935 61 ; 935 73 ;
Abstract

The present invention provides a transcriptional and translational activating sequence derived from the lambda pL transcriptional activating sequence and the E. coli lpp translational activating sequence. The activating sequence has been cloned into recombinant DNA expression vectors into which DNA sequences encoding funtional polypeptides can be readily inserted and expressed. The activating sequence of the present invention has been shown to drive high-level expression of a bovine growth hormone derivative and a human growth hormone derivative in E. coli. Preferred expression vectors of the present invention also comprise the cI857 temperature-sensitive lambda pL repressor gene, a rop.sup.- derivative of the plasmid pBR322 replicon, and a tetracycline resistance-conferring gene.


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