The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 17, 1989

Filed:

Dec. 29, 1986
Applicant:
Inventors:

Keijiro Sugimura, Osaka, JP;

Shunjiro Sugimoto, Osaka, JP;

Hounai Shirasawa, Osaka, JP;

Assignee:

Suntory Limited, Osaka, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P / ; C12R / ;
U.S. Cl.
CPC ...
435 68 ; 4351721 ; 4351723 ; 43525233 ; 4352528 ; 424 855 ;
Abstract

E. coli deficient in protease activities, said activities being not inhibited with diisopropyl fluorophosphate, phenylmethylsulfonyl fluoride, N-.alpha.-tosyl-L-lysyl chloromethyl ketone, N-tosyl-L-phenylalanine chloromethyl ketone, ethylenediamine tetra-acetic acid, leupeptin, antipain, .alpha..sub.2 -macroglobulin or chymostatin, and being inhibited with zinc chloride or copper chloride is disclosed. This E. coli is useful as a host for creating a transformant capable of expressing an exogenous protein or polypeptide. The protein or polypeptide expressed by the transformant may be extracted and purified with a quite low level of decomposition.


Find Patent Forward Citations

Sugimura, Keijiro. (1989). Novel host e. coli and use thereof (U.S. Patent No. 4874697). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/4874697

Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile

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