The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 26, 1989

Filed:

Jun. 15, 1987
Applicant:
Inventors:

Kenneth H Kortright, Cooper City, FL (US);

David E Hofheinz, Homestead, FL (US);

David M Allman, Miami, FL (US);

Meryl A Forman, Miami, FL (US);

Song Y Lee, Plantation, FL (US);

Paulette E Smariga, North Miami, FL (US);

Candie S Stoner, Hollywood, FL (US);

Assignee:

Coulter Corporation, Hialeah, FL (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N / ; G01N / ; G01N / ;
U.S. Cl.
CPC ...
435-5 ; 435-7 ; 435810 ; 436510 ; 436518 ; 436531 ; 436800 ; 436805 ; 436808 ; 436809 ; 436811 ; 436825 ; 436826 ;
Abstract

A solid-phase immunoassay is provided for determination of members of an immunological pair such as antigen and/or antibody of a single binding pair in human physiological fluid, wherein a single immunoassay enables simultaneous detection of antigen and/or antibody of a single binding pair in a test sample which may have circulating antigen and/or antibody. The immunoassay is characterized by the addition of an amount of antigen or 'spike' of the binding pair to the test sample prior to incubation of the test sample in the presence of a solid-phase absorbed antibody of the binding pair. The test sample preferably also is subjected to a lysing reagent prior to the incubation for uniformly dispensing antigens which may be present in the test sample.


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