The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 15, 1989
Filed:
Apr. 23, 1986
Applicant:
Inventors:
Assignee:
Bayer Aktiengesellschaft, Leverkusen, DE;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P / ;
U.S. Cl.
CPC ...
435 94 ; 435 97 ; 435100 ; 435174 ; 435177 ; 435178 ; 435179 ; 435180 ; 435181 ; 435182 ; 435800 ; 435813 ; 435815 ; 435819 ; 435847 ; 435880 ;
Abstract
The present invention relates to a continuous process for the enzymatic preparation of isomaltulose. A periplasmatic sucrose-mutase is produced by fermentation of microorganisms which form sucrose-mutase. The cell-free crude enzyme extract is prepared by digestion of the cells and by cross-flow microfiltration. In a single-stage, simultaneous purification and immobilization of the sucrose-mutase from the cell-free crude extract conditioned by diafiltration is obtained by selective bonding to an anionizable carrier matrix. Direct conversion of sucrose into isomaltulose is produced by the sucrose-mutase bonded to the anionizable carrier matrix, preferably in cartridge or cartouche form.