The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 27, 1989
Filed:
Jul. 11, 1986
Applicant:
Inventors:
Assignee:
Boehringer Mannheim GmbH, Mannheim-Waldhof, DE;
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N / ; C12Q / ; C12N / ;
U.S. Cl.
CPC ...
435-7 ; 435 28 ; 435188 ; 435810 ; 436512 ;
Abstract
This invention teaches a process for preparing a conjugate useful in enzyme immunoassays, and the conjugate produced by the process. The process involves oxidizing an enzyme containing a carbohydrate portion, such as peroxidase, with periodic acid or an alkali metal salt thereof in an aqueous medium followed by reduction with sodium borohydride. The oxidation and reduction step may be carried out either before or after the enzyme is coupled to the immunologically effective component of the conjugate.