The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 25, 1989
Filed:
Jul. 25, 1985
Applicant:
Inventor:
Michael J Heller, Poway, CA (US);
Assignee:
Molecular Biosystems, Inc., San Diego, CA (US);
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; G01N / ;
U.S. Cl.
CPC ...
435-6 ; 435803 ; 436501 ; 536 27 ; 935-2 ; 935-8 ; 935-9 ; 935 10 ; 935 78 ;
Abstract
An improvement in the sensitivity of hybridization assays for detecting polynucleotide target sequences is provided by selective dehybridization of the bound portion of the probe, separation of the dehybridized portion from the support and the residual sample, and concentrating the separated probe before detecting its presence by means of its reporter group. The concentration step can be carried out by adsorption of the dehybridized probe onto a basic ion exchange material. A displacer sequence having greater binding affinity for the target polynucleotide sequence may be used to dehybridize the bound probe portion for subsequent concentration.