The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 04, 1989

Filed:

Feb. 22, 1985
Applicant:
Inventor:

Nanibhushan Dattagupta, New Haven, CT (US);

Assignee:

Molecular Diagnostics, Inc., West Haven, CT (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C07H / ; C12P / ;
U.S. Cl.
CPC ...
435-6 ; 935 78 ; 536 27 ; 435 91 ; 436 94 ;
Abstract

A process for synthesizing an oligonucleotide comprising linking a nucleoside phosphate to a solid support, through the heterocyclic moiety of the nucleoside, coupling a mono- or oligonucleotide to the nucleoside phosphate through its phosphate moiety, in at least one step enzymatically lengthening the mono- or oligonucleotide, cleaving the resultant oligonucleotide from the solid support-nucleoside phosphate at the phosphate moiety of the nucleoside, and separating the oligonucleotide. After cleaving and separating the solid support-nucleoside phosphate is recycled for further coupling. Advantageously the solid support-nucleoside phosphate is phosphorylated between separation and recycling.


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